Assessment of the Cytotoxic Effects of Dimethyl Sulfoxide (DMSO) on MCF-7 and HeLa Cell Line
Keywords:
Cell Viability, Cytotoxic, DMSO, HeLa, MCF-7
Abstract
Cell culture studies on drugs and natural compounds are commonly employed for early compound screening, assessing their potential therapeutic and immunomodulatory effects in vitro. Many of these compounds have limited solubility in water and are typically dissolved in lipophilic solvents. Consequently, it is crucial to employ a biocompatible and non-toxic solvent for cell viability. Dimethyl sulfoxide (DMSO) is a widely utilized solvent in in vitro testing. This study aims to investigate the effect of DMSO on the viability of HeLa and MCF-7 cell lines. The MTT method was employed to assess cytotoxic effects on the HeLa and MCF-7 cell lines. The results revealed that concentrations of 1% and 2% DMSO significantly reduced the viability of MCF-7 cells (p<0.05) compared to the control after 24 hours of incubation. Conversely, the viability of HeLa cell lines exposed to 1% and 2% DMSO concentrations did not significantly differ from the control after 24 hours. Thus, concentrations of 1% and 2% DMSO exhibited cytotoxicity towards MCF-7 cells, while no such effect was observed in HeLa cells.
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References
Costa, L.A., Ottoni, M.H.F., Santos, M.G.D, Meireles, A.B., Almeida, V.G., Pereira, W.F., Freitas, B.A.A., Melo, G.E.A.B. (2017). Dimethyl sulfoxide (DMSO) decreases cell proliferation and TNF-α, IFN-, and IL-2 cytokine production in cultures of peripheral blood lymphocytes. Molecules. 22(11):1–10. DOI: 10.3390/molecules22111789
da Violante, G., Zerrouk, N., Richard, I., Provot, G., Chaumeil, J.C., Arnaud, P. (2002). Evaluation of the cytotoxicity effect of dimethyl sulfoxide (DMSO) on CaCo2/TC7 colon tumour cell cultures. Biol Pharm Bull. 25(12):1600–1603. DOI: 10.1248/bpb.25.1600
Ediriweera, M.K., Tennekoon, K.H., Samarakoon, S.R. (2019). In vitro assays and techniques utilized in anticancer drug discovery. J Appl Toxicol. 39(1):38–71. DOI: 10.1002/jat.3658
Forman, S., Kas, J., Fini, F., Steinberg, M., Ruml, T. (1999). The effect of different solvents on the ATP/ADP content and growth properties of HeLa cells. J Biochem Mol Toxicol. 13(1):11-5. DOI: 10.1002/(sici)1099-0461(1999)13:1<11::aid-jbt2>3.0.co;2-r
Galvao, J., Davis, B., Tilley, M., Normando, E., Duchen, M.R., Cordeiro, M.F. (2014). Unexpected low-dose toxicity of the universal solvent DMSO. FASEB J. 28(3):1317–1330. DOI: 10.1096/fj.13-235440
Jamalzadeh, L., Ghafoori, H., Sariri, R., Rabuti, H., Nasirzade, J., Hasani, H., Aghamaali, M.R. (2016). Cytotoxic effects of some common organic solvents on MCF-7, RAW-264.7 and human umbilical vein endothelial cells. Avicenna J Med Biochem. 4(1):1–6. DOI: 10.17795/ajmb-33453
Kita, H., Okamoto, K., Kushima, R., Kawauchi, A., Chano, T. (2015). Dimethyl sulfoxide induces chemotherapeutic resistance in the treatment of testicular embryonal carcinomas. Oncol Lett. 10(2):661–6. DOI: 10.3892/ol.2015.3306
Meiyanto, E., Susidarti, R.A., Handayani, S., Rahmi, F. (2008). Ekstrak etanolik biji buah pinang (Areca cathecu l.) mampu menghambat proliferasi dan memacu apoptosis sel MCF-7. Maj Farm Indones. 19(1):12–19.
Moskot, M., Jakóbkiewicz, B.J., Kloska, A., Piotrowska, E., Narajczyk, M., Gabig, C.M. (2019). The role of dimethyl sulfoxide (DMSO) in gene expression modulation and glycosaminoglycan metabolism in lysosomal storage disorders on an example of mucopolysaccharidosis. Int J Mol Sci. 20(2):1–18. DOI: 10.3390/ijms20020304
Nguyen, S.T., Nguyen, H.T.L., Truong, K.D. (2020). Comparative cytotoxic effects of methanol, ethanol and DMSO on human cancer cell lines. Biomed Res Ther, 7(7):3855–3859.
Nikolaou, N., Green, C.J., Gunn, P.J., Hodson, L., Tomlinson, J.W. (2016). Optimizing human hepatocyte models for metabolic phenotype and function: Effects of treatment with dimethyl sulfoxide (DMSO). Physiol Rep. 4(21):1–11. DOI: 10.14814/phy2.12944
Sangweni, N.F., Dludla, P.V., Chellan, N., Mabasa, L., Sharma, J.R., Johnson, R. (2021). The implication of low dose dimethyl sulfoxide on mitochondrial function and oxidative damage in cultured cardiac and cancer cells. Molecules. 26(23):7305. DOI: 10.3390/molecules26237305
Sharma, S., Raymond, E., Soda, H., Izbicka, E., Davidson, K., Lawrence, R., Von Hoff, DD. (1998). Dimethyl sulfoxide (DMSO) causes a reversible inhibition of telomerase activity in a Burkitt lymphoma cell line. Leuk Res. 22(8):663–670. DOI: 10.1016/s0145-2126(97)00188-4.
Song, Y.M., Song, S.O., Jung, Y.K., Kang, E.S., Cha, B.S., Lee, H.C., Lee, B.W. (2012). Dimethyl sulfoxide reduces hepatocellular lipid accumulation through autophagy induction. Autophagy. 8(7):1085–1097. DOI: 10.4161/auto.20260
Timm, M., Saaby, L., Moesby, L., Hansen, E.W. (2013). Considerations regarding use of solvents in in vitro cell based assays. Cytotechnology. 65(5):887–94. DOI: 10.1007/s10616-012-9530-6
Tunçer, S., Gurbanov, R., Sheraj, I., Solel, E., Esenturk, O., Banerjee, S. (2018). Low dose dimethyl sulfoxide driven gross molecular changes have the potential to interfere with various cellular processes. Sci Rep, 8(1):1–15. DOI: 10.1038/s41598-018-33234-z.
Van Tonder, A., Joubert, A.M., Cromarty, A.D. (2015). Limitations of the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-2H-tetrazolium bromide (MTT) assay when compared to three commonly used cell enumeration assays. BMC Res Notes. 8(47):1–10. DOI: 10.1186/s13104-015-1000-8.
Verheijen, M., Lienhard, M., Schrooders, Y., Clayton, O., Nudischer, R., Boerno, S., Timmermann, B., Selevsek, N., Schlapbasch, R., Gmuender, H., Gotta, S., Geraedts, J., Herwig, R., Kleinjans, J., Caiment, F. (2019). DMSO induces drastic changes in human cellular processes and epigenetic landscape in vitro. Sci Rep. 9(1):1–12. DOI: 10.1038/s41598-019-40660-0.
Vesey, D.A., Cunningham, J.M., Selden, A.C., Woodman, A.C., Hodgson, H.J.F. (1991). Dimethyl sulphoxide induces a reduced growth rate, altered cell morphology and increased epidermal-growth-factor binding in Hep G2 cells. Biochem J. 277(3):773–777. DOI: 10.1042/bj2770773
Yuan, C., Gao, J., Guo, J., Bai, L., Marshall, C., Cai, Z., Wang, L., Xiao, M. (2014). Dimethyl sulfoxide damages mitochondrial integrity and membrane potential in cultured astrocytes. PLoS One, 9(9):1–9. DOI: 10.1371/journal.pone.0107447
da Violante, G., Zerrouk, N., Richard, I., Provot, G., Chaumeil, J.C., Arnaud, P. (2002). Evaluation of the cytotoxicity effect of dimethyl sulfoxide (DMSO) on CaCo2/TC7 colon tumour cell cultures. Biol Pharm Bull. 25(12):1600–1603. DOI: 10.1248/bpb.25.1600
Ediriweera, M.K., Tennekoon, K.H., Samarakoon, S.R. (2019). In vitro assays and techniques utilized in anticancer drug discovery. J Appl Toxicol. 39(1):38–71. DOI: 10.1002/jat.3658
Forman, S., Kas, J., Fini, F., Steinberg, M., Ruml, T. (1999). The effect of different solvents on the ATP/ADP content and growth properties of HeLa cells. J Biochem Mol Toxicol. 13(1):11-5. DOI: 10.1002/(sici)1099-0461(1999)13:1<11::aid-jbt2>3.0.co;2-r
Galvao, J., Davis, B., Tilley, M., Normando, E., Duchen, M.R., Cordeiro, M.F. (2014). Unexpected low-dose toxicity of the universal solvent DMSO. FASEB J. 28(3):1317–1330. DOI: 10.1096/fj.13-235440
Jamalzadeh, L., Ghafoori, H., Sariri, R., Rabuti, H., Nasirzade, J., Hasani, H., Aghamaali, M.R. (2016). Cytotoxic effects of some common organic solvents on MCF-7, RAW-264.7 and human umbilical vein endothelial cells. Avicenna J Med Biochem. 4(1):1–6. DOI: 10.17795/ajmb-33453
Kita, H., Okamoto, K., Kushima, R., Kawauchi, A., Chano, T. (2015). Dimethyl sulfoxide induces chemotherapeutic resistance in the treatment of testicular embryonal carcinomas. Oncol Lett. 10(2):661–6. DOI: 10.3892/ol.2015.3306
Meiyanto, E., Susidarti, R.A., Handayani, S., Rahmi, F. (2008). Ekstrak etanolik biji buah pinang (Areca cathecu l.) mampu menghambat proliferasi dan memacu apoptosis sel MCF-7. Maj Farm Indones. 19(1):12–19.
Moskot, M., Jakóbkiewicz, B.J., Kloska, A., Piotrowska, E., Narajczyk, M., Gabig, C.M. (2019). The role of dimethyl sulfoxide (DMSO) in gene expression modulation and glycosaminoglycan metabolism in lysosomal storage disorders on an example of mucopolysaccharidosis. Int J Mol Sci. 20(2):1–18. DOI: 10.3390/ijms20020304
Nguyen, S.T., Nguyen, H.T.L., Truong, K.D. (2020). Comparative cytotoxic effects of methanol, ethanol and DMSO on human cancer cell lines. Biomed Res Ther, 7(7):3855–3859.
Nikolaou, N., Green, C.J., Gunn, P.J., Hodson, L., Tomlinson, J.W. (2016). Optimizing human hepatocyte models for metabolic phenotype and function: Effects of treatment with dimethyl sulfoxide (DMSO). Physiol Rep. 4(21):1–11. DOI: 10.14814/phy2.12944
Sangweni, N.F., Dludla, P.V., Chellan, N., Mabasa, L., Sharma, J.R., Johnson, R. (2021). The implication of low dose dimethyl sulfoxide on mitochondrial function and oxidative damage in cultured cardiac and cancer cells. Molecules. 26(23):7305. DOI: 10.3390/molecules26237305
Sharma, S., Raymond, E., Soda, H., Izbicka, E., Davidson, K., Lawrence, R., Von Hoff, DD. (1998). Dimethyl sulfoxide (DMSO) causes a reversible inhibition of telomerase activity in a Burkitt lymphoma cell line. Leuk Res. 22(8):663–670. DOI: 10.1016/s0145-2126(97)00188-4.
Song, Y.M., Song, S.O., Jung, Y.K., Kang, E.S., Cha, B.S., Lee, H.C., Lee, B.W. (2012). Dimethyl sulfoxide reduces hepatocellular lipid accumulation through autophagy induction. Autophagy. 8(7):1085–1097. DOI: 10.4161/auto.20260
Timm, M., Saaby, L., Moesby, L., Hansen, E.W. (2013). Considerations regarding use of solvents in in vitro cell based assays. Cytotechnology. 65(5):887–94. DOI: 10.1007/s10616-012-9530-6
Tunçer, S., Gurbanov, R., Sheraj, I., Solel, E., Esenturk, O., Banerjee, S. (2018). Low dose dimethyl sulfoxide driven gross molecular changes have the potential to interfere with various cellular processes. Sci Rep, 8(1):1–15. DOI: 10.1038/s41598-018-33234-z.
Van Tonder, A., Joubert, A.M., Cromarty, A.D. (2015). Limitations of the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-2H-tetrazolium bromide (MTT) assay when compared to three commonly used cell enumeration assays. BMC Res Notes. 8(47):1–10. DOI: 10.1186/s13104-015-1000-8.
Verheijen, M., Lienhard, M., Schrooders, Y., Clayton, O., Nudischer, R., Boerno, S., Timmermann, B., Selevsek, N., Schlapbasch, R., Gmuender, H., Gotta, S., Geraedts, J., Herwig, R., Kleinjans, J., Caiment, F. (2019). DMSO induces drastic changes in human cellular processes and epigenetic landscape in vitro. Sci Rep. 9(1):1–12. DOI: 10.1038/s41598-019-40660-0.
Vesey, D.A., Cunningham, J.M., Selden, A.C., Woodman, A.C., Hodgson, H.J.F. (1991). Dimethyl sulphoxide induces a reduced growth rate, altered cell morphology and increased epidermal-growth-factor binding in Hep G2 cells. Biochem J. 277(3):773–777. DOI: 10.1042/bj2770773
Yuan, C., Gao, J., Guo, J., Bai, L., Marshall, C., Cai, Z., Wang, L., Xiao, M. (2014). Dimethyl sulfoxide damages mitochondrial integrity and membrane potential in cultured astrocytes. PLoS One, 9(9):1–9. DOI: 10.1371/journal.pone.0107447
Published
2023-10-14
How to Cite
Suharyani, S., & Danan Jaya, B. (2023). Assessment of the Cytotoxic Effects of Dimethyl Sulfoxide (DMSO) on MCF-7 and HeLa Cell Line. JURNAL MEDIA KESEHATAN, 16(1), 1-8. https://doi.org/https://doi.org/10.33088/jmk.v16i1.926
